Search results for "Gap gene"

showing 4 items of 4 documents

Practicing logical reasoning through Drosophila segmentation gene mutants.

2021

Laboratory practical sessions are critical to scientific training in biology but usually fail to promote logical and hypothesis-driven reasoning and rely heavily on the teacher's instructions. This paper describes a 2-day laboratory practicum in which students prepare and analyze larval cuticle preparations of Drosophila segmentation gene mutant strains. Embryonic segmentation involves three major classes of genes according to their loss-of-function phenotypes: the establishment of broad regions by gap genes, the specification of the segments by the pair-rule genes, and the compartments within segments by the segment polarity genes. Students are asked to sort undefined segmentation mutants …

0303 health sciencesLogical reasoningeducation05 social sciencesMutant050301 educationPracticumGene Expression Regulation DevelopmentalComputational biologyBiologyBiochemistry03 medical and health sciencesSegmentation geneSegment polarity genePhenotypeLogical conjunctionAnimalsHumansSegmentationDrosophila0503 educationMolecular BiologyGap gene030304 developmental biologyBiochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular BiologyREFERENCES
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Ems and Nkx6 are central regulators in dorsoventral patterning of the Drosophila brain

2009

In central nervous system development, the identity of neural stem cells (neuroblasts) critically depends on the precise spatial patterning of the neuroectoderm in the dorsoventral (DV) axis. Here, we uncover a novel gene regulatory network underlying DV patterning in the Drosophila brain, and show that the cephalic gap gene empty spiracles (ems) and the Nk6 homeobox gene (Nkx6) encode key regulators. The regulatory network implicates novel interactions between these and the evolutionarily conserved homeobox genes ventral nervous system defective (vnd), intermediate neuroblasts defective (ind) and muscle segment homeobox (msh). We show that Msh cross-repressively interacts with Nkx6 to sust…

Nervous systemEmbryo Nonmammaliananimal structuresBiologyNeuroblastmedicineAnimalsDrosophila ProteinsMolecular BiologyGap geneBody PatterningHomeodomain ProteinsGeneticsRegulation of gene expressionNeuroectodermNeural tubeBrainGene Expression Regulation DevelopmentalCell biologymedicine.anatomical_structureVentral nerve cordembryonic structuresHomeoboxDrosophilaTranscription FactorsDevelopmental BiologyDevelopment
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Reverse-engineering post-transcriptional regulation of gap genes in Drosophila melanogaster

2013

16 páginas, 6 figuras, 1 tabla

Systems biologyContext (language use)Computational biology03 medical and health sciencesCellular and Molecular Neuroscience0302 clinical medicineKrüppelGeneticsAnimalsDrosophila ProteinsRNA MessengerMolecular BiologyPost-transcriptional regulationlcsh:QH301-705.5Ecology Evolution Behavior and SystematicsGap gene030304 developmental biologyGenetics0303 health sciencesEcologybiologyModels GeneticProtein StabilitySystems BiologyGene Expression Regulation Developmentalbiology.organism_classificationRepressor ProteinsDrosophila melanogasterComputational Theory and Mathematicslcsh:Biology (General)Modeling and SimulationIdentifiabilityDrosophila melanogasterGenetic Engineering030217 neurology & neurosurgeryDrosophila ProteinResearch Article
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Molecular markers for identified neuroblasts in the developing brain of Drosophila.

2003

The Drosophila brain develops from the procephalic neurogenic region of the ectoderm. About 100 neural precursor cells (neuroblasts) delaminate from this region on either side in a reproducible spatiotemporal pattern. We provide neuroblast maps from different stages of the early embryo (stages 9, 10 and 11, when the entire population of neuroblasts has formed), in which about 40 molecular markers representing the expression patterns of 34 different genes are linked to individual neuroblasts. In particular, we present a detailed description of the spatiotemporal patterns of expression in the procephalic neuroectoderm and in the neuroblast layer of the gap genes empty spiracles, hunchback, hu…

animal structuresFasciclin 2EctodermBiologyNeuroblastmedicineMorphogenesisAnimalsDrosophila ProteinsMolecular BiologyGap geneIn Situ HybridizationGeneticsHomeodomain ProteinsNeuronsNeuroectodermfungiGenes HomeoboxBrainGene Expression Regulation DevelopmentalNuclear ProteinsNeuromereCell biologyDNA-Binding Proteinsmedicine.anatomical_structureDrosophila melanogasternervous systemembryonic structuresTrans-ActivatorsHomeotic geneGanglion mother cellBiomarkersDevelopmental BiologyDevelopment (Cambridge, England)
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